Hybrid combinatorial chemistry strategies that use DNA as an information-carrying medium are proving to be powerful\r\ntools for molecular discovery. In order to extend these efforts, we present a highly parallel format for DNA-programmed\r\nchemical library synthesis. The new format uses a standard microwell plate footprint and is compatible with commercially\r\navailable automation technology. It can accommodate a wide variety of combinatorial synthetic schemes with up to 384\r\ndifferent building blocks per chemical step. We demonstrate that fluidic routing of DNA populations in the highly parallel\r\nformat occurs with excellent specificity, and that chemistry on DNA arrayed into 384 well plates proceeds robustly, two\r\nrequirements for the high-fidelity translation and efficient in vitro evolution of small molecules.
Loading....